Journal: Journal of Neurochemistry

Article Title: A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis

doi: 10.1111/jnc.12029

Figure Lengend Snippet: Competition radioligand binding on tsA201 cells transiently expressing with human α7 nicotinic acetylcholine receptors (nAChRs). Equilibrium radioligand binding was performed with [ 3 H]-α-bungarotoxin (1 nM). Spinosad caused no significant displacement of [ 3 H]-α-bungarotoxin binding, whereas MLA caused complete displacement of specific radioligand binding ( K i = 4.2 ± 0.2 nM). Data points are means of triplicate samples (± SEM) from a single experiment, and data are typical of three independent experiments.

Article Snippet: Radioligand binding to transiently transfected tsA201 cells was performed essentially as described previously (Lansdell and Millar ).

Techniques: Binding Assay, Expressing

Journal: Cell Reports

Article Title: The α 2 δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α 2 δ-1

doi: 10.1016/j.celrep.2018.10.033

Figure Lengend Snippet:

Article Snippet: For co-IPs and electrophysiological studies, tsA-201 cells were transfected using Fugene6 (Promega, Fitchburg, WI) according to the manufacturer’s protocol.

Techniques: Affinity Purification, Recombinant, Modification, Nucleic Acid Electrophoresis, Membrane, Plasmid Preparation, Bradford Assay, Mutagenesis, Software