Journal: Scientific Reports

Article Title: Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression

doi: 10.1038/s41598-023-40347-7

Figure Lengend Snippet: Sortilin (SORT) interacts with progranulin (PRGN) and LPL in PCa cells and PRGN is secreted by aggressive PC3 cells. ( a ) Representative confocal images showing co-labelling of cells with anti-PRGN (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( b ) Quantification of colocalisation between PRGN and SORT. ( c ) Amount of PRGN in conditioned media (CM) and corresponding cell lysates with quantification of band densities and ratio of PRGN in CM to PRGN in cell lysates. ( d ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and PRGN was detected by Western blotting. Input; cell lysate. ( e ) Representative confocal images showing co-labelling of cells with anti-LPL (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( f ) Quantification of colocalisation between LPL and SORT. ( g ) Amount of LPL in CM and corresponding cell lysates with quantification of band densities in cell lysates. In ( c ) and ( g ) PNT2 bands from the same membrane were shifted towards LNCaP and PC3 bands. Western blotting signal was normalised using total protein staining. ( h ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and LPL was detected by Western blotting. Input; cell lysate. Data are presented as mean ± SD, in ( b , f ) n = 10 representative ROIs, in ( c , g ) n = 3 (independent experiments), one-way ANOVA.

Article Snippet: Human prostate cell PNT2 (ECACC 95012613), LNCaP (clone FCG, ECACC 89110211) and PC3 (ECACC 90112714) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) from CellBank Australia (Children's Medical Research Institute, Westmead, NSW, Australia).

Techniques: Immunoprecipitation, Western Blot, Membrane, Staining

Journal: Journal of Neurochemistry

Article Title: A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis

doi: 10.1111/jnc.12029

Figure Lengend Snippet: Competition radioligand binding on tsA201 cells transiently expressing with human α7 nicotinic acetylcholine receptors (nAChRs). Equilibrium radioligand binding was performed with [ 3 H]-α-bungarotoxin (1 nM). Spinosad caused no significant displacement of [ 3 H]-α-bungarotoxin binding, whereas MLA caused complete displacement of specific radioligand binding ( K i = 4.2 ± 0.2 nM). Data points are means of triplicate samples (± SEM) from a single experiment, and data are typical of three independent experiments.

Article Snippet: Radioligand binding to transiently transfected tsA201 cells was performed essentially as described previously (Lansdell and Millar ).

Techniques: Binding Assay, Expressing

Journal: Cell Reports

Article Title: The α 2 δ-like Protein Cachd1 Increases N-type Calcium Currents and Cell Surface Expression and Competes with α 2 δ-1

doi: 10.1016/j.celrep.2018.10.033

Figure Lengend Snippet:

Article Snippet: For co-IPs and electrophysiological studies, tsA-201 cells were transfected using Fugene6 (Promega, Fitchburg, WI) according to the manufacturer’s protocol.

Techniques: Affinity Purification, Recombinant, Modification, Nucleic Acid Electrophoresis, Membrane, Plasmid Preparation, Bradford Assay, Mutagenesis, Software